Mol. Microbiol
Research & Publication
Research
분자미생물 연구실에서는 전 세계적으로 급성호흡기 질환 사망률 1위를 차지하고 있으며 수막염, 중이염 등의 주된 원인균인 폐렴구균(Streptococcus Pneumoniae)의 감염면역에 대한 분자생물학적인 연구를 수행중이다. 특히 폐렴구균이 숙주에 침입할 때 나타나는 병원균-숙주 상호작용 기전을 규명하고자 한다.
1) 폐렴구균이 열 충격, 영양 변화 등의 스트레스에 노출될 때 유도되는 스트레스 단백질의 발현 조절 및 기능에 대한 분자생물학적 연구.
2) 폐렴구균 숙주 침입 시 유도되는 숙주 유전자 발현 및 이의 발현 조절에 의한 치료제/백신 개발.
3) 특정 유전자에 대한 염증 조절제/치료제에 대한 작용 기전 연구.
4) 천연물질인 홍삼을 이용한 뇌수막염 치료 기전 연구등을 수행중이다.
이러한 연구 결과를 바탕으로 본 연구실에서는 병원균-숙주 간의 상호작용 교란을 통한 치료제/백신 개발에 대한 연구를 수행하고 있다.
연구분야
- 본 연구실에서 개발한 폐렴구균 변이주는 인후감염은 물론 뇌 및 혈액 내로 직접 주입해도 생쥐가 전혀 치사되지 않으며 면역기능 결핍 생쥐 (Nude & SCID mouse)에 주입해도 안전하였다. 따라서 이를 이용한 새로운 인후점막백신을 개발하고자 한다.
- 현재까지 숙주세포의 ATF3는 cytokine 분비를 억제한다고 알려졌으나 본 연구자는 그람양성균인 폐렴구균 감염 시 cytokine 분비가 촉진됨을 발견하였다. 따라서 본 연구를 통해 폐렴구균 감염 시 유도되는 ATF3가 어떻게 cytokine 분비를 조절하여 염증을 유도하는지를 규명하고자 한다.
- 또한 폐렴구균이 숙주 침투 시 발현되는 유전자들의 특성을 규명함으로써, 폐렴구균과 숙주 상호 작용을 규명하고자 한다.
Publication
Stress responses in Streptococcus species and their effects on the host / Oct 2015 · The Journal of Microbiology
Panax ginseng aqueous extract prevents pneumococcal sepsis in vivo by potentiating cell survival and diminishing inflammation / Aug 2015 · Phytomedicine
Theobromine inhibits differentiation of 3T3-L1 cells during the early stage of adipogenesis via AMPK and MAPK signaling pathways / Article · Jun 2015
Allicin inhibits invasion and migration of breast cancer cells through the suppression of VCAM-1: Regulation of association between p65 and ER-α / May 2015 · Journal of Functional Foods
Involvement of ATF3 in the negative regulation of iNOS expression and NO production in activated macrophages / Mar 2015 · Immunologic Research
ClpL is a chaperone without auxiliary factor / Feb 2015 · FEBS Journal
TLR4 Mediates Pneumolysin-Induced ATF3 Expression through the JNK/p38 Pathway in Streptococcus pneumoniae-Infected RAW 264.7 Cells / Jan 2015 · Molecules and Cells
A bivalent conjugate vaccine containing PspA families 1 and 2 has the potential to protect against a wide range of Streptococcus pneumoniae strains and Salmonella Typhi / Dec 2014 · Vaccine
Ethanol-Induced Alcohol Dehydrogenase E (AdhE) Potentiates Pneumolysin in Streptococcus pneumoniae / Oct 2014 · Infection and Immunity
Preparation and testing of a Vi conjugate vaccine using pneumococcal surface protein A (PspA) from Streptococcus pneumoniae as the carrier protein / Aug 2014 · Vaccine
Adenylate kinase from Streptococcus pneumoniae is essential for growth through its catalytic activity / Jul 2014 · FEBS Open Bio
Streptococcus pneumoniae ClpL Modulates Adherence to A549 Human Lung Cells through Rap1/Rac1 Activation / Jun 2014 · Infection and immunity
ATF3 Confers Resistance to Pneumococcal Infection Through Positive Regulation of Cytokine Production / Jun 2014 · The Journal of Infectious Diseases
Korean Red Ginseng inhibits apoptosis in neuroblastoma cells via estrogen receptor β-mediated PI3K/AKT signaling / Jun 2014 · Journal of ginseng research
Chinese Yam Extracts Containing β‐Sitosterol and Ethyl Linoleate Protect against Atherosclerosis in Apolipoprotein E‐Deficient Mice and Inhibit Muscular Expression of VCAM‐1 In Vitro / Apr 2014 · Journal of Food Science
Ethanol induces cell cycle arrest and triggers apoptosis via Sp1-dependent p75NTR expression in human neuroblastoma cells/ Sep 2013 · Cell Biology and Toxicology
Kobophenol A enhances proliferation of human osteoblast-like cells with activation of the p38 pathway / Sep 2013 · International immunopharmacology
Modified Opsonization, Phagocytosis, and Killing Assays To Measure Potentially Protective Antibodies against Pneumococcal Surface Protein A / Aug 2013 · Clinical and vaccine Immunology: CVI
Antistress effect of red ginseng in brain cells is mediated by TACE repression via PADI4 / Jul 2013 · Journal of ginseng research
Anti-oxidative Stress Effect of Red Ginseng in the Brain is Mediated by Peptidyl Arginine Deiminase Type IV (PADI4) Repression via Estrogen Receptor (ER) β Up-regulation. / May 2013 · Journal of ethnopharmacology
Streptococcus pneumoniae ClpP protease induces apoptosis via caspase-independent pathway in human neuroblastoma cells: Cytoplasmic relocalization of p53 / May 2013 · Toxicon
Inactivated pep27 mutant as an effective mucosal vaccine against a secondary lethal pneumococcal challenge in mice / Jan 2013 · Clinical and Experimental Vaccine Research
Pneumococcal Surface Protein A Inhibits Complement Deposition on the Pneumococcal Surface by Competing with the Binding of C-Reactive Protein to Cell-Surface Phosphocholine / Oct 2012 · The Journal of Immunology
Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk / Jan 2012 · Korean Journal of Veterinary Research
Stereocalpin A inhibits the expression of adhesion molecules in activated vascular smooth muscle cells / Dec 2011 · International immunopharmacology
Streptococcus pneumoniae pep27 mutant as a live vaccine for serotype-independent protection in mice / Dec 2011 · Vaccine
Sulforaphane suppresses vascular adhesion molecule-1 expression in TNF-α-stimulated mouse vascular smooth muscle cells: Involvement of the MAPK, NF-κB and AP-1 signaling pathways / Dec 2011 · Vascular Pharmacology
Korean Red Ginseng Up-regulates C21-Steroid Hormone Metabolism via Cyp11a1 Gene in Senescent Rat Testes / Sep 2011 · Journal of ginseng research
Heat-Shock Protein ClpL/HSP100 Increases Penicillin Tolerance in Streptococcus pneumoniae / Aug 2011 · Advances in Oto-Rhino-Laryngology
Decrease in Penicillin Susceptibility Due to Heat Shock Protein ClpL in Streptococcus pneumoniae / Mar 2011 · Antimicrobial Agents and Chemotherapy
Inhibitory Effects of Ginsenoside Rb1 on Atopic Dermatitis-Like Skin Lesions in Mice / Dec 2010 · Journal of ginseng research
ATF3 plays a key role in Kdo2-Lipid A-induced TLR4-Dependent Gene Expression via NF-κB activation / Dec 2010 · PLoS ONE
Comparative In Vitro Activities of Torezolid (DA-7157) against Clinical Isolates of Aerobic and Anaerobic Bacteria in South Korea / Dec 2010 · Antimicrobial Agents and Chemotherapy
Inhibition of TNF-α-induced adhesion molecule expression by diosgenin in mouse vascular smooth muscle cells via downregulation of the MAPK, Akt and NF-κB signaling pathways / Oct 2010 · Vascular Pharmacology
The inhibitory effect of acrylamide on NCAM expression in human neuroblastoma cells: Involvement of CK2/Ikaros signaling pathway / Oct 2010 · Toxicology in Vitro
Anti-apoptotic Effects of Red Ginseng on Oxidative Stress Induced by Hydrogen Peroxide in SK-N-SH Cells / Jun 2010 · Journal of ginseng research
Virulence attenuation of Streptococcus pneumoniae clpP mutant by sensitivity to oxidative stress in macrophages via an NO-mediated pathway / Apr 2010 · The Journal of Microbiology
Enhancement of Murine NK cell Activity in vitro by Red Ginseng Acidic Polysaccharide / Dec 2009 · Journal of ginseng research
The role of activating transcription factor 3 in stress-induced macrophage apoptosis and senescence / Oct 2009 · Cytokine
Characterization of ASC-2 as an Antiatherogenic Transcriptional Coactivator of Liver X Receptors in Macrophages / May 2009 · Molecular Endocrinology
Characterization of the Streptococcus pneumoniae BgaC Protein as a Novel Surface -Galactosidase with Specific Hydrolysis Activity for the Gal 1-3GlcNAc Moiety of Oligosaccharides / Apr 2009 · Journal of bacteriology
Reduction-sensitive and cysteine residue-mediated Streptococcus pneumoniae HrcA oligomerization in vitro / Mar 2009 · Molecules and Cells
Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: Involvement of Ikaros phosphorylation by casein kinase 2 / Oct 2008 · Cellular and Molecular Life Sciences CMLS
Red ginseng acidic polysaccharide (RGAP) in combination with IFN-gamma results in enhanced macrophage function through activation of the NF-kappaB pathway. / Aug 2008 · Bioscience Biotechnology and Biochemistry
Red Ginseng Acidic Polysaccharide (RGAP) in Combination with IFN-γ Results in Enhanced Macrophage Function through Activation of the NF-κB Pathway / Jul 2008 · Bioscience Biotechnology and Biochemistry
Antibacterial and anti-atrophic effects of a highly soluble, acid stable UDCA formula in Helicobacter pylori-induced gastritis / Jul 2008 · Biochemical pharmacolog
y
Dual regulation of dnaK and groE operons by HrcA and Ca++ in Streptococcus pneumoniae / May 2008 · Archives of Pharmacal Research
Selective toxicity of ginsenoside Rg3 on multidrug resistant cells by membrane fluidity modulation / Mar 2008 · Archives of Pharmacal Research
Effects of Panax ginseng on Stress / Jan 2008 · Journal of ginseng research
Prodigiosin isolated from Hahella chejuensis suppresses lipopolysaccharide-induced NO production by inhibiting p38 MAPK, JNK and NF-κB activation in murine peritoneal macrophages / Jan 2008 · International Immunopharmacology
Erratum for Le et al., Modulation of Adherence, Invasion, and Tumor Necrosis Factor Alpha Secretion during the Early Stages of Infection by Streptococcus pneumoniae ClpL / Jun 2007 · Infection and Immunity
Differential regulation of the genes of the Streptococcus pneumoniae dnaK operon by Ca++ / May 2007 · Molecules and Cells
Reversal of multidrug resistance by 4-chloro-N-(3-((E)-3-(4-hydroxy-3-methoxyphenyl)acryloyl)phenyl)benzamid e through the reversible inhibition of P-glycoprotein / Apr 2007 · Biochemical and Biophysical Research Communications
Molecular Epidemiology of Community-Associated Antimicrobial-Resistant Staphylococcus aureus in Seoul, Korea (2003): Pervasiveness of Multidrug-Resistant SCC mec Type II Methicillin-Resistant S. aureus / Feb 2007 · Microbial Drug Resistance
Effects of Non-Saponin Red Ginseng Components on Multi-drug Resistance / Jan 2007 · Journal of ginseng research
Inhibition of ICAM-1 expression by garlic component, allicin, in gamma-irradiated human vascular endothelial cells via downregulation of the JNK signaling pathway / Jan 2007 · International Immunopharmacology
Gamma-irradiation-induced Intercellular Adhesion Molecule-1 (ICAM-1) Expression is Associated with Catalase: Activation of Ap-1 and JNK / Jan 2007 · Journal of Toxicology and Environmental Health Part A
Analyses of single nucleotide polymorphisms and haplotype linkage of the human ABCB1 (MDR1) gene in Korean / Jan 2007 · Archives of Pharmacal Research
Allicin, a major component of garlic, inhibits apoptosis of macrophage in a depleted nutritional state / Nov 2006 · Nutrition
Regulation of Insulin Secretion and -Cell Mass by Activating Signal Cointegrator 2 / Jul 2006 · Molecular and Cellular Biology
Hydrophobic surface exposure-mediated CIRCE repression by the Ca2+-sensor, HrcA, in Streptococcus pneumoniae / Apr 2006 · International Congress Series
Methamphetamine Administration Produces Immunomodulation in Mice / Jan 2006 · Journal of Toxicology and Environmental Health Part A
Immunomodulatory function of murine NK cell activity by alginate / Dec 2005 · Archives of Pharmacal Research
Comparison of the rabbit pyrogen test and Limulus amoebocyte lysate (LAL) assay for endotoxin in hepatitis B vaccines and the effect of aluminum hydroxide / Oct 2005 · Biologicals
Caspase-independent cell death by allicin in human epithelial carcinoma cells: Involvement of PKA / Article · Jul 2005 · Cancer Letters
Ca2+-dependent of the CIRCE regulon in Streptococcus pneumoniae / Feb 2005 · Molecular Microbiology
Biological Activities and Chemistry of Saponins from Panax ginseng C. A. Meyer / Jan 2005 · Phytochemistry Reviews
Modulation of murine macrophage function by methamphetamine / Dec 2004 · Journal of Toxicology and Environmental Health Part A
The ClpP Protease of Streptococcus pneumoniae Modulates Virulence Gene Expression and Protects against Fatal Pneumococcal Challenge / Nov 2004 · Infection and Immunity
Vitamin C blocks TNF-alpha-induced NF-kappaB activation and ICAM-1 expression in human neuroblastoma cells. / Nov 2004 · Archives of Pharmacal Research
Optimized Culture Conditions for Production of the chimaeric protein, Uropathogenic Escherichia coli Adhesin - Cholera Toxin A2B Subunits, in Escherichia coli TB1 / Jan 2004 · Biomolecules and Therapeutics
Induction of apoptosis and expression of apoptosis related genes in human epithelial carcinoma cells by Helicobacter pylori VacA toxin / Dec 2003 · Toxicon
Antiviral and tumoricidal activities of alginate-stimulated macrophages are mediated by different mechanisms / Dec 2003 · Archives of Pharmacal Research
Effect of Heat Shock and Mutations in ClpL and ClpP on Virulence Gene Expression in Streptococcus pneumoniae / Aug 2003 · Infection and Immunity
Immune Alterations in Mice Exposed to the Herbicide Simazine / Jul 2003 · Journal of
Toxicology and Environmental Health Part A
Modulation of TNF-α-induced ICAM-1 expression, NO and H2O2 production by alginate, allicin and ascorbic acid in human endothelial cells / Apr 2003 · Archives of Pharmacal Research
Reversal of p-glycoprotein-mediated multidrug resistance by ginsenosides Rg(3). Biochem Pharmacol / Feb 2003 · Biochemical Pharmacology
The immunomodulatory effects of the herbicide simazine on murine macrophage functions in vitro. Toxicol In Vitro / Nov 2002 · Toxicology in Vitro
Immunostimulating effects of acidic polysaccharides extract of Panax ginseng on macrophage function / Sep 2002 · Immunopharmacology and Immunotoxicology
Korea Red Ginseng Alters Electroencephalogram Spectra of Sleep-Wake Stage in Rats / Aug 2008 · J. Ginseng Res.
Enzymatic biosynthesis of cephalexin / Jun 1980 · Biotechnology and Bioengineering
Genetic transformation in Streptococcus pneumoniae: Molecular cloning and characterization of recP, a gene required for genetic recombination / Mar 1988 · Journal of Bacteriology
Genetic transformation in Streptococcus pneumoniae: Nucleotide sequence and predicted amino acid sequence of recP / Aug 1990 · Journal of Bacteriology
Antiviral and antitumoral activities of domestic medicinal plants in macrophages / Jan 1995 · 생약학회지
Characterization of several transformation-deficient mutants of Streptococcus pneumoniae in DNA damage / Jan 1995 · Archives of Pharmacal Research
Instability of pneumococcus library in pHC79 and pACYC184 / Feb 1995 · Archives of Pharmacal Research
Optimization of culture conditions for production of pneumococcal capsular polysaccharide type IV / May 1996 · Archives of Pharmacal Research
Effects of ginseng saponin on modulation of multidrug resistance / Jun 1996 · Archives of Pharmacal Research
High Incidence of Multidrug-Resistant Streptococcus pneumoniae in South Korea / Dec 1996 · Microbial Drug Resistance
Characterization of the Stress Response in Streptococcus pneumoniae / Feb 1997 · Advances in Experimental Medicine and Biology
Effects of the administration of Bezoar Bovis on immune responses of mice / Jan 1998 · 생약학회지
Evaluation of the immunotoxicity of herbicide simazine in mice / Mar 1998 · Faseb Journal
Molecular cloning, expression, and characterization of dnaK in Streptococcus pneumoniae / May 1998 · FEMS Microbiology Letters
Isolation of a multidrug resistance inhibitor fromAconitum pseudo-laeve var.erectum / Jul 1998 · Archives of Pharmacal Research
Aflatoxin B1-induced suppression of nitric oxide production in murine peritoneal macrophages / Jan 1999 · Journal of Toxicology and Environmental Health Part A
Limited Stress Response in Streptococcus pneumoniae / Feb 1999 · Microbiology and Immunology
Inhibition of various functions in murine peritoneal macrophages by aflatoxin B1 exposure in vivo / Feb 1999 · International Journal of Immunopharmacology
Involvement of NO, H2O2 and TNF-α in the reduced antitumor activity of murine peritoneal macrophages by aflatoxin B1 / Apr 1999 · Cancer Letters
Development of anti-corrosive and wear resistant copper alloy for bushings / Apr 1999 · JSAE Review
In vitro suppressive effect of aflatoxin B1 on murine peritoneal macrophage functions / May 1999 · Toxicology
Identification of the Polyamine Induced Proteins in Escherichia coli / May 1999 · Molecules and Cells
Antitumor activity of 4-phenyl-1-arylsulfonylimidazolidinone, DW2143 / Jul 1999 · Cancer Letters
Enhanced macrophage antitumor effects of protein A in combination with IFN-γ / Jul 1999 · Archives of Pharmacal Research
Characterization of dnaK Mutants in Streptococcus pneumoniae / Jan 2000 · BMB reports
Characterization of dnaK mutants in Streptococcus pneumoniae / Jan 2000 · Journal of biochemistry and molecular biology
Activation of Murine Peritoneal Macrophages by Streptococcus pneumoniae Type II Capsular Polysaccharide: Involvement of CD14-Dependent Pathway / Aug 2000 · Scandinavian Journal of Immunology
Alteration of kinase-mediated signalings in murine peritoneal macrophages by aflatoxin B1 / Aug 2000 · Cancer Letters
Stimulation of various functions in murine peritoneal macrophages by high mannuronic acid-containing alginate (HMA) exposure in vivo / Feb 2001 · International Immunopharmacology
Molecular cloning and characterization of groESL operon in Streptococcus pneumoniae / Jul 2001 · Molecules and Cells
Inhibition of γ-Irradiation Induced Adhesion Molecules and NO Production by Alginate in Human Endothelial Cells / Nov 2001 · Archives of Pharmacal Research
Involvement of protein kinase C and tyrosin kinase in tumoricidal activation of macrophage induced by Streptococcus pneumoniae type II capsular polysaccharide / Feb 2002 · International Immunopharmacology